Fig. 7. Effects of Nox inhibitor on the FIR-induced ROS and EMT signaling. (A) HCT116 p53⁺/⁺ cells received FIR with or without Nox inhibitor DPI treated 1 h prior to the last irradiation. Intracellular ROS levels were determined by FACS analysis for indicated time points. Results show representative data of independent experiments performed in triplicate wells (*, p ≤ 0.05; **, p ≤ 0.01). (B and C) Cells pretreated with DPI or NAC were analyzed by immunoblotting for the expression of EMT markers and associated signaling molecules as described.